Human acute lymphocytes
[Cell number]
[Cell abbreviation]
Cell
[Cell name]
Human Acute lymphocytes
[Background information]
See cell instructions
[Pay attention to the digestion time when digesting cells. Insufficient digestion will cause damage when pipetting the cells. Generally, The digestion time is minutes, but it is based on the fact that most of the cells retract into a spherical shape during microscopic examination. Generally, the cells can be pipetted down in a few times. If the digestion is not enough, cell pipetting may damage the cells. Cell line cryopreservation solution formula B+, if the adherent cells are unevenly distributed and grow into islands, human acute lymphocytes can digest the cells, resuspend and break up the cells, and add fresh medium for culture]
The main source of cells is B and a few well-known domestic and foreign universities established departments
[Generation]
[Specifications]
One bottle of recovery culture bottle or frozen Two cryopreservation tubes
[Number of cells]
*
[Biosafety level]
[Organism]
Human
[Tissue source]
Organ peripheral blood disease acute lymphoblastic leukemia endothelium
[Cell morphology]
Lymphocytes
[Cell Characteristics]
Suspension
[Special attention should be paid to if you are using a public laboratory or are new to cell culture. It is recommended to add double-antibody culture. Adherent cells should not be cultured on the day they are received. Digest immediately. Please change the medium of the cells and place them in an incubator to incubate until the next day before digestion and passage. Please give priority to the diameter of the culture dish for passage. If the cells are adherent cells and are suspended or partially suspended when received, status, please centrifuge the suspended cells immediately, add serum-based complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with serum-based complete medium for culture for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up to solve the problem]
bbr B
[Cell Test] < br/>The cells do not contain Mycoplasma B bacteria, yeast and fungi
[Culture conditions]
See the cell instructions for details
[Passaging method]
Two recommended Change the medium once a day
[Freezing conditions]
See cell instructions for details
[Main literature]
Please refer to relevant published articles for details
Precautions when purchasing cells
After receiving the cells, first observe whether the cell bottle is intact and whether the culture medium is leaking or turbid. If the above phenomena occur, please contact us in time.
Read the cell instructions carefully to understand the cell-related information, such as cell morphology, medium, serum ratio, required cytokines, etc.
Wipe the surface of the cell bottle with alcohol and observe the cell status under a microscope. Due to transportation problems, a small amount of adherent cells will fall off the bottle wall. Place the cells in an incubator for overnight culture, and then take them out for observation the next day. Human acute lymphocytes
At this time, most cells will adhere to the wall. If the cells still cannot adhere, please use trypan blue staining to determine the cell viability. If the cell viability is confirmed to be normal, please centrifuge the cells and reattach them with fresh culture medium. If the staining results of wall culture show that the cells are inactive, please take a photo and contact us in time. After the information is confirmed, we will send it to you again for free.
It is recommended that customers take a few photos of the cells the day before receiving them to record the status of the cells to facilitate communication with the company's technical department.
Cell passaging procedures
1. Passaging of adherent cells
Place culture medium B in a ℃ water bath to preheat in advance, wipe it with alcohol, and then place it on a clean bench.
Aspirate or pour out the old culture medium in the cell bottle, and add a small amount of B to rinse the cells.
Add an appropriate amount of trypsin so that the amount of trypsin can cover the cells, incubate at ℃, observe under a microscope every ~, discard when the gaps between adherent cells become larger and the cells tend to be round but have not floated. Trypsin, add fresh culture medium, shake the cell flask to stop the trypsin action.
Use a pipette to carefully pipette the adherent cells to make a cell suspension. Control the strength of the blowing to avoid creating a large number of bubbles.
Human acute lymphocytes
Inoculate the cell suspension into another ~ cell bottles, add fresh culture medium, culture in a ℃ incubator, and observe the adherent growth the next day.
2. Passaging of Suspension Cells
Transfer the cell suspension to a sterile centrifuge tube and centrifuge.
Discard the supernatant, add fresh culture medium, and carefully blow off the precipitate with a pipette to make a cell suspension.
Inoculate the cell suspension into another ~ cell bottles, add fresh culture medium, and culture in a ℃ incubator.
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, human acute lymphocytes
The company provides background information Organism growth characteristics Source organ type Morphology Culture conditions Application system Disease tissue cryopreservation conditions, etc. Recovery and cryopreservation instructions Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
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, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
B cell lines
, the company provides background information on organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell With extensive experience in cell culture, we can provide a full range of technical support for cell culture, making your experiments hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information human acute lymphocytes
Organism growth characteristics source organ type morphology culture conditions application system disease tissue cryopreservation conditions, etc. recovery and cryopreservation instructions Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
Cell lines
, the company provides background information, organism growth characteristics, source, organ type, morphology, culture conditions, application, disease tissue cryopreservation conditions, recovery and cryopreservation instructions information, we have a wealth of cell lines Culture experience, can provide all-round technical support for cell culture, so that your experiments will be hassle-free!
1301; human acute T lymphocytes